TGFβ1 inhibits lymphatic endothelial cell differentiation from mouse embryonic stem cells
Identifieur interne : 000239 ( France/Analysis ); précédent : 000238; suivant : 000240TGFβ1 inhibits lymphatic endothelial cell differentiation from mouse embryonic stem cells
Auteurs : Daniel Vittet [France] ; Galina Merdzhanova [France] ; Marie-Hélène Prandini [France] ; Jean-Jacques Feige [France] ; Sabine Bailly [France]Source :
- Journal of Cellular Physiology [ 0021-9541 ] ; 2012-11.
Abstract
The lymphatic vasculature is essential for the maintenance of tissue fluid, immune surveillance, and dissemination of metastasis. Recently, several models for lymphatic vascular research and markers specific for lymphatic endothelium have been characterized. Despite these significant achievements, our understanding of the early lymphatic development is still rather limited. The purpose of the study was to further define early lymphatic differentiation regulatory pathways. In the present study, we have developed conditions leading to lymphatic endothelial cell differentiation under both serum‐rich and serum‐free conditions, using the coculture system of Flk‐1‐positive vascular precursors derived from murine embryonic stem (ES) cells grown on an OP9 stromal cell layer. In this work, we also identified Transforming Growth Factor‐β1 (TGFβ1) as a negative regulator of lymphvasculogenesis from ES‐derived vascular progenitors. Finally, we could show that TGFβ1 addition decreases COUP‐TFII and Sox18 mRNA levels, which are two transcription factors known to be involved in early lymphatic endothelial differentiation. Taken together these findings support the concept that manipulating the TGFβ signaling pathway may represent an interesting target to favor lymphatic endothelial cell expansion for cell replacement strategies. J. Cell. Physiol. 227: 3593–3602, 2012. © 2012 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/jcp.24063
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 005C30
- to stream Istex, to step Curation: 005C30
- to stream Istex, to step Checkpoint: 000352
- to stream Main, to step Merge: 004680
- to stream Main, to step Curation: 004655
- to stream Main, to step Exploration: 004655
- to stream France, to step Extraction: 000239
Links to Exploration step
ISTEX:C4D37925D3FCE9FD2D09E1DA3079E282CE0DFA00Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">TGFβ1 inhibits lymphatic endothelial cell differentiation from mouse embryonic stem cells</title><author><name sortKey="Vittet, Daniel" sort="Vittet, Daniel" uniqKey="Vittet D" first="Daniel" last="Vittet">Daniel Vittet</name></author><author><name sortKey="Merdzhanova, Galina" sort="Merdzhanova, Galina" uniqKey="Merdzhanova G" first="Galina" last="Merdzhanova">Galina Merdzhanova</name></author><author><name sortKey="Prandini, Marie Elene" sort="Prandini, Marie Elene" uniqKey="Prandini M" first="Marie-Hélène" last="Prandini">Marie-Hélène Prandini</name></author><author><name sortKey="Feige, Jean Acques" sort="Feige, Jean Acques" uniqKey="Feige J" first="Jean-Jacques" last="Feige">Jean-Jacques Feige</name></author><author><name sortKey="Bailly, Sabine" sort="Bailly, Sabine" uniqKey="Bailly S" first="Sabine" last="Bailly">Sabine Bailly</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C4D37925D3FCE9FD2D09E1DA3079E282CE0DFA00</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1002/jcp.24063</idno><idno type="url">https://api.istex.fr/document/C4D37925D3FCE9FD2D09E1DA3079E282CE0DFA00/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">005C30</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">005C30</idno><idno type="wicri:Area/Istex/Curation">005C30</idno><idno type="wicri:Area/Istex/Checkpoint">000352</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000352</idno><idno type="wicri:doubleKey">0021-9541:2012:Vittet D:tgf:inhibits:lymphatic</idno><idno type="wicri:Area/Main/Merge">004680</idno><idno type="wicri:Area/Main/Curation">004655</idno><idno type="wicri:Area/Main/Exploration">004655</idno><idno type="wicri:Area/France/Extraction">000239</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">TGFβ1 inhibits lymphatic endothelial cell differentiation from mouse embryonic stem cells</title><author><name sortKey="Vittet, Daniel" sort="Vittet, Daniel" uniqKey="Vittet D" first="Daniel" last="Vittet">Daniel Vittet</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U1036, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CEA, DSV, iRTSV, Laboratoire Biologie du Cancer et de l'Infection, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Université Joseph Fourier, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement><settlement type="city">Grenoble</settlement></placeName><orgName type="university">Université Joseph Fourier</orgName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Inserm U1036, Laboratoire BCI, iRTSV, CEA Grenoble, 17 rue des martyrs, 38054 Grenoble Cedex 9</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation></author><author><name sortKey="Merdzhanova, Galina" sort="Merdzhanova, Galina" uniqKey="Merdzhanova G" first="Galina" last="Merdzhanova">Galina Merdzhanova</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U1036, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CEA, DSV, iRTSV, Laboratoire Biologie du Cancer et de l'Infection, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Université Joseph Fourier, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement><settlement type="city">Grenoble</settlement></placeName><orgName type="university">Université Joseph Fourier</orgName></affiliation></author><author><name sortKey="Prandini, Marie Elene" sort="Prandini, Marie Elene" uniqKey="Prandini M" first="Marie-Hélène" last="Prandini">Marie-Hélène Prandini</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U1036, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CEA, DSV, iRTSV, Laboratoire Biologie du Cancer et de l'Infection, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Université Joseph Fourier, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement><settlement type="city">Grenoble</settlement></placeName><orgName type="university">Université Joseph Fourier</orgName></affiliation></author><author><name sortKey="Feige, Jean Acques" sort="Feige, Jean Acques" uniqKey="Feige J" first="Jean-Jacques" last="Feige">Jean-Jacques Feige</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U1036, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CEA, DSV, iRTSV, Laboratoire Biologie du Cancer et de l'Infection, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Université Joseph Fourier, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement><settlement type="city">Grenoble</settlement></placeName><orgName type="university">Université Joseph Fourier</orgName></affiliation></author><author><name sortKey="Bailly, Sabine" sort="Bailly, Sabine" uniqKey="Bailly S" first="Sabine" last="Bailly">Sabine Bailly</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U1036, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CEA, DSV, iRTSV, Laboratoire Biologie du Cancer et de l'Infection, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Université Joseph Fourier, Grenoble</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Grenoble</settlement><settlement type="city">Grenoble</settlement></placeName><orgName type="university">Université Joseph Fourier</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Cellular Physiology</title><title level="j" type="alt">JOURNAL OF CELLULAR PHYSIOLOGY</title><idno type="ISSN">0021-9541</idno><idno type="eISSN">1097-4652</idno><imprint><biblScope unit="vol">227</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="3593">3593</biblScope><biblScope unit="page" to="3602">3602</biblScope><biblScope unit="page-count">10</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2012-11">2012-11</date></imprint><idno type="ISSN">0021-9541</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9541</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The lymphatic vasculature is essential for the maintenance of tissue fluid, immune surveillance, and dissemination of metastasis. Recently, several models for lymphatic vascular research and markers specific for lymphatic endothelium have been characterized. Despite these significant achievements, our understanding of the early lymphatic development is still rather limited. The purpose of the study was to further define early lymphatic differentiation regulatory pathways. In the present study, we have developed conditions leading to lymphatic endothelial cell differentiation under both serum‐rich and serum‐free conditions, using the coculture system of Flk‐1‐positive vascular precursors derived from murine embryonic stem (ES) cells grown on an OP9 stromal cell layer. In this work, we also identified Transforming Growth Factor‐β1 (TGFβ1) as a negative regulator of lymphvasculogenesis from ES‐derived vascular progenitors. Finally, we could show that TGFβ1 addition decreases COUP‐TFII and Sox18 mRNA levels, which are two transcription factors known to be involved in early lymphatic endothelial differentiation. Taken together these findings support the concept that manipulating the TGFβ signaling pathway may represent an interesting target to favor lymphatic endothelial cell expansion for cell replacement strategies. J. Cell. Physiol. 227: 3593–3602, 2012. © 2012 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Grenoble</li></settlement><orgName><li>Université Joseph Fourier</li></orgName></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Vittet, Daniel" sort="Vittet, Daniel" uniqKey="Vittet D" first="Daniel" last="Vittet">Daniel Vittet</name></region><name sortKey="Bailly, Sabine" sort="Bailly, Sabine" uniqKey="Bailly S" first="Sabine" last="Bailly">Sabine Bailly</name><name sortKey="Bailly, Sabine" sort="Bailly, Sabine" uniqKey="Bailly S" first="Sabine" last="Bailly">Sabine Bailly</name><name sortKey="Bailly, Sabine" sort="Bailly, Sabine" uniqKey="Bailly S" first="Sabine" last="Bailly">Sabine Bailly</name><name sortKey="Feige, Jean Acques" sort="Feige, Jean Acques" uniqKey="Feige J" first="Jean-Jacques" last="Feige">Jean-Jacques Feige</name><name sortKey="Feige, Jean Acques" sort="Feige, Jean Acques" uniqKey="Feige J" first="Jean-Jacques" last="Feige">Jean-Jacques Feige</name><name sortKey="Feige, Jean Acques" sort="Feige, Jean Acques" uniqKey="Feige J" first="Jean-Jacques" last="Feige">Jean-Jacques Feige</name><name sortKey="Merdzhanova, Galina" sort="Merdzhanova, Galina" uniqKey="Merdzhanova G" first="Galina" last="Merdzhanova">Galina Merdzhanova</name><name sortKey="Merdzhanova, Galina" sort="Merdzhanova, Galina" uniqKey="Merdzhanova G" first="Galina" last="Merdzhanova">Galina Merdzhanova</name><name sortKey="Merdzhanova, Galina" sort="Merdzhanova, Galina" uniqKey="Merdzhanova G" first="Galina" last="Merdzhanova">Galina Merdzhanova</name><name sortKey="Prandini, Marie Elene" sort="Prandini, Marie Elene" uniqKey="Prandini M" first="Marie-Hélène" last="Prandini">Marie-Hélène Prandini</name><name sortKey="Prandini, Marie Elene" sort="Prandini, Marie Elene" uniqKey="Prandini M" first="Marie-Hélène" last="Prandini">Marie-Hélène Prandini</name><name sortKey="Prandini, Marie Elene" sort="Prandini, Marie Elene" uniqKey="Prandini M" first="Marie-Hélène" last="Prandini">Marie-Hélène Prandini</name><name sortKey="Vittet, Daniel" sort="Vittet, Daniel" uniqKey="Vittet D" first="Daniel" last="Vittet">Daniel Vittet</name><name sortKey="Vittet, Daniel" sort="Vittet, Daniel" uniqKey="Vittet D" first="Daniel" last="Vittet">Daniel Vittet</name><name sortKey="Vittet, Daniel" sort="Vittet, Daniel" uniqKey="Vittet D" first="Daniel" last="Vittet">Daniel Vittet</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000239 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000239 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:C4D37925D3FCE9FD2D09E1DA3079E282CE0DFA00
|texte= TGFβ1 inhibits lymphatic endothelial cell differentiation from mouse embryonic stem cells
}}
| This area was generated with Dilib version V0.6.31. |  |